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讓基因體技術真正落地

不是實驗的起點，也不是答案的終點，
但我們讓兩者連成一線。

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2025.01 與菲律賓 Manila HealthTek 簽訂代理合約
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技術服務

技術服務



次世代定序( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

RAD-seq限制酶點位核酸定序



RAD-seq：簡化基因體，從建庫到分析

限制酶點位核酸定序服務（RAD-seq，Restriction-site Associated DNA Sequencing），以限制酶簡化基因體資訊，提升有效數據比例，可同時處理大量族群樣本，降低研究負擔。RAD-seq 可應用於無參考序列之目標物種的族群遺傳變異、定義個體間遺傳差異、族群親緣地理關係追溯等生物演化研究；同時適用於單核苷酸多態性（SNP，Single Nucleotide Polymorphism）分子標誌建立，可有效應用於農林漁牧等相關產業。


RAD-seq 技術核心
    • RAD-seq 是結合了限制酶處理和高通量定序優勢的簡化基因體學技術，用於檢測與分析基因體間的遺傳變異。
    • 以限制酶切位和片段長度為篩選條件，使 RAD-seq 無需對整個基因體進行定序，降低定序深度需求，同時提高樣品間相似片段的有效序列定序比例。
    • RAD-seq 具有多樣品混合定序的特性，使用 DNA 標籤技術（DNA barcoding），可同時研究大量樣本，快速處理個體/族群間的基因體變異，獲得 SNPs 資訊。
    • 技術廣泛應用於研究自然種群中的遺傳多樣性、物種分化、突變和進化，以及全基因關聯分析（GWAS）和數量性狀基因座定位（QTL mapping）等研究領域。

RAD-seq 建庫介紹
    • Digestion & Ligation:
      • 利用限制酶將 DNA 切割為較小的片段，針對其切位將 DNA 片段標記上特定標籤序列，每個樣本都有唯一的標記序列，用以區分來自不同樣本的片段。
    • Pooling:
      • 將來自不同樣本的 DNA 片段混合在一起，形成 DNA 樣品庫。
    • Size Selection:
      • 經由 Size Selection 保留適合上機定序的片段長度，同時去除過短的片段。
    • PCR:
      • 使用 PCR 反應增殖 DNA 片段，同時完成 DNA 樣品庫的製備，準備進行 NovaSeq 高通量定序。
    • Sequencing:
      • 完成定序後，對序列進行比對、組裝和分析，獲取樣本中基因體變異的資訊，得到 SNPs 資訊。



標準服務


您只需要提供DNA樣品，即可享有樣品建庫、定序，以及生物資訊組裝服務。組裝後的數據可直接供後續各項分析使用，免除研究人員實驗前置作業、數據組裝與處理格式問題所耗時間及設備成本。





進階/客製化分析服務

艾達特提供 RAD-seq 組裝後的衍生分析，可輸出符合指定期刊格式之科學圖表。依照需求客製化製圖，強調分析成果亮點，並提供兩次修改與重分析服務，確保分析內容符合研究探討主軸，可提供親緣關係樹分析（Phylogenetic Tree）、族群遺傳結構分析（Population genetic structure）、主成分分析（PCA/DAPC）等，除上列進階分析項目，我們也提供客製化分析服務，可依照您提供的參考文獻進行分析評估，如基因隔離地圖（Dispersal map of resistance）、遺傳分化係數分析（Fixation index analysis）、全基因組關聯分析（GWAS）或數量性狀基因座定位（QTL mapping）等。



技術諮詢/樣品測試服務

在您決定使用艾達特的服務之前，您可以聯繫我們，讓我們為您進行 RAD-seq 的基礎介紹以及研究、應用方向的討論，確保艾達特提供的服務能契合您的期望，同時提供給您樣品測試的服務，透過少量的樣品進行酵素適用性測試，確認您的目標樣品所適合的製備方式。


第三代定序( Nanopore )


What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

客製化服務


次世代定序( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

RAD-seq：簡化基因體，從建庫到分析

限制酶點位核酸定序服務（RAD-seq，Restriction-site Associated DNA Sequencing），以限制酶簡化基因體資訊，提升有效數據比例，可同時處理大量族群樣本，降低研究負擔。RAD-seq 可應用於無參考序列之目標物種的族群遺傳變異、定義個體間遺傳差異、族群親緣地理關係追溯等生物演化研究；同時適用於單核苷酸多態性（SNP，Single Nucleotide Polymorphism）分子標誌建立，可有效應用於農林漁牧等相關產業。


RAD-seq 技術核心
    • RAD-seq 是結合了限制酶處理和高通量定序優勢的簡化基因體學技術，用於檢測與分析基因體間的遺傳變異。
    • 以限制酶切位和片段長度為篩選條件，使 RAD-seq 無需對整個基因體進行定序，降低定序深度需求，同時提高樣品間相似片段的有效序列定序比例。
    • RAD-seq 具有多樣品混合定序的特性，使用 DNA 標籤技術（DNA barcoding），可同時研究大量樣本，快速處理個體/族群間的基因體變異，獲得 SNPs 資訊。
    • 技術廣泛應用於研究自然種群中的遺傳多樣性、物種分化、突變和進化，以及全基因關聯分析（GWAS）和數量性狀基因座定位（QTL mapping）等研究領域。

RAD-seq 建庫介紹
    • Digestion & Ligation:
      • 利用限制酶將 DNA 切割為較小的片段，針對其切位將 DNA 片段標記上特定標籤序列，每個樣本都有唯一的標記序列，用以區分來自不同樣本的片段。
    • Pooling:
      • 將來自不同樣本的 DNA 片段混合在一起，形成 DNA 樣品庫。
    • Size Selection:
      • 經由 Size Selection 保留適合上機定序的片段長度，同時去除過短的片段。
    • PCR:
      • 使用 PCR 反應增殖 DNA 片段，同時完成 DNA 樣品庫的製備，準備進行 NovaSeq 高通量定序。
    • Sequencing:
      • 完成定序後，對序列進行比對、組裝和分析，獲取樣本中基因體變異的資訊，得到 SNPs 資訊。



標準服務


您只需要提供DNA樣品，即可享有樣品建庫、定序，以及生物資訊組裝服務。組裝後的數據可直接供後續各項分析使用，免除研究人員實驗前置作業、數據組裝與處理格式問題所耗時間及設備成本。





進階/客製化分析服務

艾達特提供 RAD-seq 組裝後的衍生分析，可輸出符合指定期刊格式之科學圖表。依照需求客製化製圖，強調分析成果亮點，並提供兩次修改與重分析服務，確保分析內容符合研究探討主軸，可提供親緣關係樹分析（Phylogenetic Tree）、族群遺傳結構分析（Population genetic structure）、主成分分析（PCA/DAPC）等，除上列進階分析項目，我們也提供客製化分析服務，可依照您提供的參考文獻進行分析評估，如基因隔離地圖（Dispersal map of resistance）、遺傳分化係數分析（Fixation index analysis）、全基因組關聯分析（GWAS）或數量性狀基因座定位（QTL mapping）等。



技術諮詢/樣品測試服務

在您決定使用艾達特的服務之前，您可以聯繫我們，讓我們為您進行 RAD-seq 的基礎介紹以及研究、應用方向的討論，確保艾達特提供的服務能契合您的期望，同時提供給您樣品測試的服務，透過少量的樣品進行酵素適用性測試，確認您的目標樣品所適合的製備方式。


第三代定序( Nanopore )


What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications



次世代定序( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

RAD-seq限制酶點位核酸定序



RAD-seq：簡化基因體，從建庫到分析

限制酶點位核酸定序服務（RAD-seq，Restriction-site Associated DNA Sequencing），以限制酶簡化基因體資訊，提升有效數據比例，可同時處理大量族群樣本，降低研究負擔。RAD-seq 可應用於無參考序列之目標物種的族群遺傳變異、定義個體間遺傳差異、族群親緣地理關係追溯等生物演化研究；同時適用於單核苷酸多態性（SNP，Single Nucleotide Polymorphism）分子標誌建立，可有效應用於農林漁牧等相關產業。


RAD-seq 技術核心
    • RAD-seq 是結合了限制酶處理和高通量定序優勢的簡化基因體學技術，用於檢測與分析基因體間的遺傳變異。
    • 以限制酶切位和片段長度為篩選條件，使 RAD-seq 無需對整個基因體進行定序，降低定序深度需求，同時提高樣品間相似片段的有效序列定序比例。
    • RAD-seq 具有多樣品混合定序的特性，使用 DNA 標籤技術（DNA barcoding），可同時研究大量樣本，快速處理個體/族群間的基因體變異，獲得 SNPs 資訊。
    • 技術廣泛應用於研究自然種群中的遺傳多樣性、物種分化、突變和進化，以及全基因關聯分析（GWAS）和數量性狀基因座定位（QTL mapping）等研究領域。

RAD-seq 建庫介紹
    • Digestion & Ligation:
      • 利用限制酶將 DNA 切割為較小的片段，針對其切位將 DNA 片段標記上特定標籤序列，每個樣本都有唯一的標記序列，用以區分來自不同樣本的片段。
    • Pooling:
      • 將來自不同樣本的 DNA 片段混合在一起，形成 DNA 樣品庫。
    • Size Selection:
      • 經由 Size Selection 保留適合上機定序的片段長度，同時去除過短的片段。
    • PCR:
      • 使用 PCR 反應增殖 DNA 片段，同時完成 DNA 樣品庫的製備，準備進行 NovaSeq 高通量定序。
    • Sequencing:
      • 完成定序後，對序列進行比對、組裝和分析，獲取樣本中基因體變異的資訊，得到 SNPs 資訊。



標準服務


您只需要提供DNA樣品，即可享有樣品建庫、定序，以及生物資訊組裝服務。組裝後的數據可直接供後續各項分析使用，免除研究人員實驗前置作業、數據組裝與處理格式問題所耗時間及設備成本。





進階/客製化分析服務

艾達特提供 RAD-seq 組裝後的衍生分析，可輸出符合指定期刊格式之科學圖表。依照需求客製化製圖，強調分析成果亮點，並提供兩次修改與重分析服務，確保分析內容符合研究探討主軸，可提供親緣關係樹分析（Phylogenetic Tree）、族群遺傳結構分析（Population genetic structure）、主成分分析（PCA/DAPC）等，除上列進階分析項目，我們也提供客製化分析服務，可依照您提供的參考文獻進行分析評估，如基因隔離地圖（Dispersal map of resistance）、遺傳分化係數分析（Fixation index analysis）、全基因組關聯分析（GWAS）或數量性狀基因座定位（QTL mapping）等。



技術諮詢/樣品測試服務

在您決定使用艾達特的服務之前，您可以聯繫我們，讓我們為您進行 RAD-seq 的基礎介紹以及研究、應用方向的討論，確保艾達特提供的服務能契合您的期望，同時提供給您樣品測試的服務，透過少量的樣品進行酵素適用性測試，確認您的目標樣品所適合的製備方式。


第三代定序( Nanopore )


What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

客製化服務


次世代定序( Illumina )

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

客製化服務

關於艾達特

關於艾達特

我們以「艾達特」命名並成立公司，概念取自於定序過程中Adaptor（轉接子）的作用， 期許我們能在生物與資訊領域中扮演承接與轉化的角色，成為學術界與產業界在樣品庫前端製備、 後端數據分析的最佳合作夥伴，讓彼此在快速變遷的時代中，能以適存者 (adaptor) 之姿克服挑戰，共創無限的可能。

發展目標

艾達特致力於建構「客戶導向之基因體技術整合服務平台」，自前端樣品製備建庫至後端數據處理分析提供系列化服務，旨在開啟新型態基因體技術於臺灣之廣泛應用。本公司現透過與高雄醫學大學產學合作，強化基因體科學以及產業應用鏈結，藉以提高服務平台各環節之整合性與完整度，進而依照客戶之學術亦或產業需求，為其量身打造客製化分析流程，跳脫一體適用的業界訴求，以「理解需求，購買所求」模式，精準突破當前生物科學市場困境。
客製化諮詢服務

客製化諮詢服務

提供基因體技術諮詢，依照您希望應用的技術項目進行應用說明， 幫助您了解即將進行的服務品項與分析結果，
銜接您的計畫、 目標與本公司所提供的服務品項，並客製化您所享有的基因體服務。

技術應用諮詢

以本公司所提供之RAD-seq、RNA 基因體技術服務品項為主，提供實驗前討論諮詢。

生物資訊諮詢

依照您所取得的生物資訊數據，進行分析前諮詢，確保您所使用的基因體服務符合您的需求。

客製化數據分析

本公司同時提供您DNA、RNA的客製化分析，可依照您提供的參考文獻進行分析，分析如親緣關係樹建立、遺傳結構分析等。
艾達特團隊介紹

艾達特團隊介紹

邱奕凡

執行長
生物資訊部總監

李菡勻

營運長
技術研發部總監

萬郁岑

研發工程師
生物資訊部門

李沂晏

數據分析師
生物資訊部門
聯絡我們

聯絡我們

地址

807413 高雄市三民區民族一路80號2樓之1(B10)

連絡電話

07-9769339

聯絡信箱

生物資訊部 yfchiu@adaptor-genosci.com
營運管理部 hyli@adaptor-genosci.com

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