艾達特,專注於串接樣本與數據的每一步。

我們是連結生物與資訊的轉接子,讓基因體技術真正落地。

不是實驗的起點,也不是答案的終點,

但我們讓兩者連成一線。



2025.01.15 與菲律賓 Manila HealthTek 簽訂代理合約

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成立年

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經手樣品數

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經手物種種類

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國內外送案單位


技術服務

技術服務


Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning

Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions


Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning

Short-Read Sequencing ( Illumina )

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning
關於艾達特

關於艾達特

我們以「艾達特」命名並成立公司,概念取自於定序過程中Adaptor(轉接子)的作用, 期許我們能在生物與資訊領域中扮演承接與轉化的角色,成為學術界與產業界在樣品庫前端製備、 後端數據分析的最佳合作夥伴,讓彼此在快速變遷的時代中,能以適存者 (adaptor) 之姿克服挑戰,共創無限的可能。

發展目標

艾達特致力於建構「客戶導向之基因體技術整合服務平台」,自前端樣品製備建庫至後端數據處理分析提供系列化服務,旨在開啟新型態基因體技術於臺灣之廣泛應用。本公司現透過與高雄醫學大學產學合作,強化基因體科學以及產業應用鏈結,藉以提高服務平台各環節之整合性與完整度,進而依照客戶之學術亦或產業需求,為其量身打造客製化分析流程,跳脫一體適用的業界訴求,以「理解需求,購買所求」模式,精準突破當前生物科學市場困境。
客製化諮詢服務

客製化諮詢服務

提供基因體技術諮詢,依照您希望應用的技術項目進行應用說明, 幫助您了解即將進行的服務品項與分析結果,
銜接您的計畫、 目標與本公司所提供的服務品項,並客製化您所享有的基因體服務。

技術應用諮詢

以本公司所提供之RAD-seq、RNA 基因體技術服務品項為主,提供實驗前討論諮詢。

生物資訊諮詢

依照您所取得的生物資訊數據,進行分析前諮詢,確保您所使用的基因體服務符合您的需求。

客製化數據分析

本公司同時提供您DNA、RNA的客製化分析,可依照您提供的參考文獻進行分析,分析如親緣關係樹建立、遺傳結構分析等。
艾達特團隊介紹

艾達特團隊介紹

邱奕凡

執行長
生物資訊部總監

李菡勻

營運長
技術研發部總監

萬郁岑

研發工程師
生物資訊部門

李沂晏

數據分析師
生物資訊部門
聯絡我們

聯絡我們

地址

80708高雄市三民區民族一路80號B10辦公室2樓之1(B10)

連絡電話

07-9769339

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生物資訊部 yfchiu@adaptor-genosci.com
營運管理部 hyli@adaptor-genosci.com

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