艾達特,專注於串接樣本與數據的每一步。
我們是連結生物與資訊的轉接子,讓基因體技術真正落地。
不是實驗的起點,也不是答案的終點,
但我們讓兩者連成一線。

2025.01.15 與菲律賓 Manila HealthTek 簽訂代理合約
0
成立年
0
經手樣品數
0
經手物種種類
0
國內外送案單位
技術服務
技術服務
Sanger Sequencing
What is Amplicon Sequencing?
- Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
- Ideal for analyzing known sequences with high accuracy and reliability.
📋 Workflow
- Primer design
- PCR amplification of target region
- Purification and Sanger sequencing
- Sequence analysis and comparison
🎯 Applications
- Mutation detection and SNP validation
- Species identification (e.g., COI, 16S)
- Microbial strain analysis
- CRISPR editing verification
- Sequence confirmation in molecular cloning
⭐ Key Benefits
- High accuracy
- Suitable for small sample numbers
- Cost-effective and easy to perform
What is Plasmid Sequencing?
- Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
- Typically performed using universal primers or custom-designed primers.
📋 Workflow
- Plasmid DNA extraction
- Primer selection (e.g., M13, T7, or custom)
- Sanger sequencing reaction
- Sequence analysis and verification
🎯 Applications
- Validate gene construct accuracy
- Confirm insert sequence and orientation
- Detect mutations or splice sites
- Prepare sequences for database submission (e.g., GenBank)
⭐ Key Benefits
- High accuracy with ~600–1000 bp read length
- Suitable for small inserts or full plasmid sequencing
- Fast, easy, and cost-effective
Short-Read Sequencing ( Illumina )
What is Amplicon Sequencing (Short-Read)?
- Uses PCR to amplify specific target regions of DNA.
- Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
- Suitable for analyzing specific genes or markers across many samples.
📋 Workflow
- Primer design (target-specific)
- PCR amplification of target regions
- Library preparation and indexing
- Illumina short-read sequencing
- Data analysis and variant calling
🎯 Applications
- Microbiome profiling (e.g., 16S/ITS)
- Pathogen detection
- Genetic variation and SNP analysis
- Environmental DNA (eDNA) studies
- Cancer hotspot mutation screening
⭐ Key Benefits
- High-throughput and cost-efficient
- Ideal for multiplexing large sample sets
- High sensitivity for low-frequency variants
- Suitable for targeted regions up to ~600 bp
What is Plasmid Sequencing (Short-Read)?
- Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
- Ideal for full plasmid verification or multiplexed plasmid analysis.
📋 Workflow
- Plasmid DNA extraction
- Fragmentation and library preparation
- Illumina short-read sequencing
- Assembly and sequence verification
🎯 Applications
- Full plasmid sequence confirmation
- Detection of point mutations, indels, or rearrangements
- High-throughput screening of plasmid libraries
- QC for plasmid-based gene therapy or vaccine vectors
⭐ Key Benefits
- Ultra-high accuracy and depth
- Suitable for large plasmids and pooled samples
- Allows full plasmid reconstruction
- Scalable and automation-friendly
What is mRNA-seq?
- Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
- Captures a snapshot of active genes in cells or tissues.
📋 Workflow
- RNA extraction
- mRNA enrichment or rRNA depletion
- cDNA synthesis and library preparation
- Illumina sequencing
- Transcript quantification and analysis
🎯 Applications
- Differential gene expression analysis
- Biomarker discovery
- Pathway and functional analysis
- Disease mechanism research (e.g., cancer, infection)
- Drug response and toxicogenomics
⭐ Key Benefits
- High sensitivity and reproducibility
- Suitable for a wide range of samples
- Enables whole-transcriptome analysis
- Scalable for large sample numbers
What is Whole Exome Sequencing (WES)?
- Targets and sequences all protein-coding regions (exons) of the human genome.
- Covers ~1–2% of the genome but contains ~85% of known disease-related variants.
📋 Workflow
- Genomic DNA extraction
- Exome capture/enrichment
- Library preparation and Illumina sequencing
- Variant calling and annotation
🎯 Applications
- Rare disease gene discovery
- Cancer genomics and somatic mutation analysis
- Inherited disease and carrier screening
- Personalized and precision medicine
⭐ Key Benefits
- Focused on clinically relevant regions
- Cost-effective compared to whole-genome sequencing
- High coverage and accuracy
- Scalable for large cohort studies
What is Whole Genome Sequencing (WGS)?
- Sequences the entire human genome, including coding and non-coding regions.
- Provides the most comprehensive view of genetic variation.
📋 Workflow
- Genomic DNA extraction
- Library preparation
- Illumina short-read sequencing
- Read alignment and variant analysis
🎯 Applications
- Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
- Population genetics and evolutionary studies
- Disease gene discovery
- Cancer genomics and precision medicine
⭐ Key Benefits
- Covers the entire genome
- High data quality and depth
- Ideal for discovery-based research
- Supports large-scale studies and biobanks
What is Shotgun Metagenomic Sequencing?
- Randomly sequences all DNA in a sample to profile entire microbial communities.
- Provides insights into both taxonomy and functional potential (genes, pathways).
📋 Workflow
- DNA extraction from environmental or biological samples
- Library preparation
- Illumina short-read sequencing
- Taxonomic classification and functional annotation
🎯 Applications
- Microbiome profiling (gut, soil, marine, etc.)
- Detection of pathogens and antimicrobial resistance genes
- Environmental and industrial microbial studies
- Functional analysis of complex communities
⭐ Key Benefits
- Species- and strain-level resolution
- Simultaneous detection of bacteria, viruses, fungi, and more
- Functional gene profiling (not just "who", but "what they do")
- Culture-independent and unbiased
What is RAD-seq?
- A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
- Efficient for genotyping across the genome without sequencing the whole genome.
📋 Workflow
- Genomic DNA digestion with restriction enzymes
- Adapter ligation and PCR amplification
- Library preparation and Illumina sequencing
- SNP discovery and genotyping
🎯 Applications
- Population genetics and phylogenetics
- Linkage mapping and QTL analysis
- Genomic selection and marker development
- Studies of non-model organisms
⭐ Key Benefits
- Cost-effective genome-wide variant discovery
- Works without a reference genome
- Suitable for large numbers of samples
- High-resolution genotyping for evolutionary studies
Long-Read Sequencing ( Nanopore / PacBio )
Nanopore
What is Long-Read WGS?
- Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
- Enables accurate assembly and detection of complex variants across the entire genome.
📋 Workflow
- High-molecular-weight DNA extraction
- Library preparation (no PCR required)
- Nanopore long-read sequencing
- Read alignment and genome analysis
🎯 Applications
- Structural variant detection (SVs, CNVs, inversions)
- De novo genome assembly
- Disease gene discovery and genome phasing
- Cancer genomics and epigenetic analysis (e.g., methylation)
⭐ Key Benefits
- Ultra-long reads for resolving complex regions
- PCR-free workflow preserves native DNA modifications
- Detects variants missed by short-read methods
- Enables full haplotype phasing and improved assembly
What is Long-Read Plasmid Sequencing?
- Uses Nanopore sequencing to read entire plasmid molecules in a single run.
- Provides complete, circularized plasmid sequences without assembly gaps.
📋 Workflow
- Plasmid DNA extraction (high purity)
- Library preparation (minimal fragmentation)
- Nanopore long-read sequencing
- Circular assembly and variant analysis
🎯 Applications
- Full plasmid verification (including insert, backbone, and junctions)
- Detection of mutations, rearrangements, or contamination
- Characterization of gene therapy or vaccine vectors
- Synthetic biology construct validation
⭐ Key Benefits
- Complete plasmid reconstruction in one read
- High accuracy and structural resolution
- PCR-free workflow preserves native sequence context
- Fast turnaround and scalable for multiple constructs
What is Microbial WGS (Long-Read)?
- Uses Nanopore sequencing to generate complete microbial genomes in single runs.
- Enables accurate assembly of chromosomes, plasmids, and mobile elements.
📋 Workflow
- High-quality microbial DNA extraction
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- De novo assembly and genome annotation
🎯 Applications
- Complete microbial genome reconstruction
- Identification of plasmids, phages, and resistance genes
- Comparative genomics and strain typing
- Pathogen surveillance and outbreak tracing
⭐ Key Benefits
- Resolves repetitive regions and structural variants
- Generates finished, circular genomes
- Culture-independent and fast
- Ideal for clinical, environmental, or industrial strains
What is Long-Read Amplicon Sequencing?
- Uses Nanopore sequencing to read full-length PCR amplicons.
- Enables sequencing of long or complex regions that are difficult for short-read platforms.
📋 Workflow
- Target-specific PCR amplification
- Barcoding and library preparation
- Nanopore long-read sequencing
- Consensus building and variant analysis
🎯 Applications
- Full-length 16S/ITS rRNA gene profiling
- HLA genotyping and immune repertoire analysis
- Detection of structural variants in specific genes
- Pathogen identification and strain-level resolution
⭐ Key Benefits
- Long reads cover entire amplicons in single reads
- No assembly required, less bias
- High-resolution taxonomic and genetic analysis
- Scalable and multiplex-friendly
What is Long-Read Metagenomic Sequencing?
- Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
- Captures complete microbial genomes and resolves complex communities.
📋 Workflow
- DNA extraction from environmental or clinical samples
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- Taxonomic and functional analysis
🎯 Applications
- Microbiome analysis at species or strain level
- Detection of antimicrobial resistance genes and plasmids
- Viral, bacterial, and fungal pathogen identification
- Real-time monitoring of environmental or clinical samples
⭐ Key Benefits
- Full-length reads enable species- and strain-level resolution
- No PCR bias; captures low-abundance or novel organisms
- Enables genome reconstruction from metagenomic data
- Fast and portable sequencing for field applications
PacBio
What is PacBio Amplicon Sequencing?
- Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
- Ideal for complex or repetitive regions, and for distinguishing closely related variants.
📋 Workflow
- Target-specific PCR amplification
- SMRTbell library preparation
- PacBio long-read sequencing (HiFi reads)
- Consensus sequence generation and analysis
🎯 Applications
- Full-length 16S/ITS rRNA profiling
- HLA typing and immune repertoire analysis
- Detection of rare variants and gene isoforms
- Amplicon-based microbial community studies
⭐ Key Benefits
- High accuracy (HiFi reads ≥99.9%)
- Full-length reads without assembly
- Resolves complex or repetitive regions
- Ideal for highly similar sequences or low-frequency variants
Clinical Testing Services/Reagents
What is Pharmacogenetic qPCR Testing?
- Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
- Based on real-time PCR for rapid and reliable genotyping.
📋 Workflow
- Sample collection (e.g., buccal swab or blood)
- DNA extraction
- qPCR amplification with gene-specific probes
- Genotype analysis and interpretation
🎯 Applications
- Personalized medicine and drug response prediction
- Dose adjustment for anticoagulants, antidepressants, etc.
- Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
- Reducing risk of adverse drug reactions
⭐ Key Benefits
- Fast, accurate, and cost-effective
- Compatible with clinical sample types
- Easy-to-use format for clinical labs
- Enables precision treatment decisions
What is TSO500?
- A comprehensive NGS panel for analyzing 500+ cancer-related genes.
- Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.
📋 Workflow
- DNA/RNA extraction from FFPE or fresh samples
- Library preparation and hybrid capture
- Illumina sequencing (NextSeq/NovaSeq)
- Bioinformatic analysis and clinical interpretation
🎯 Applications
- Comprehensive tumor profiling
- Identification of actionable mutations and drug targets
- Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
- Support for precision oncology and immunotherapy decisions
⭐ Key Benefits
- All-in-one assay for solid tumor characterization
- High sensitivity and specificity
- Compatible with clinical sample types (FFPE)
- Enables personalized cancer treatment strategies
What is Hereditary Disease Testing?
- Analyzes inherited genetic variants associated with hereditary disorders.
- Helps assess disease risk, carrier status, and guide family planning.
📋 Workflow
- Sample collection (blood or saliva)
- DNA extraction and library preparation
- NGS or panel-based sequencing
- Variant interpretation and clinical reporting
🎯 Applications
- Screening for inherited cancer syndromes (e.g., BRCA1/2)
- Carrier testing for autosomal recessive disorders
- Genetic diagnosis of rare diseases
- Family risk assessment and genetic counseling
⭐ Key Benefits
- Early detection and prevention
- Supports personalized treatment decisions
- Informative for reproductive planning
- Comprehensive panels for multiple conditions
Others
What is Oligo Synthesis?
- Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
- Customized for specific research, diagnostic, or therapeutic purposes.
📋 Workflow
- Sequence design and order placement
- Automated phosphoramidite synthesis
- Deprotection and purification
- Quality control (e.g., MALDI-TOF, OD260)
🎯 Applications
- PCR, qPCR, and RT-PCR primers
- Probes for molecular diagnostics
- Gene editing (e.g., CRISPR guide RNAs)
- siRNA, antisense, or aptamer research
⭐ Key Benefits
- High purity and customization options
- Fast turnaround and scalable production
- Modified bases, labels, and delivery formats available
- Reliable support for research and clinical needs
What is Whole Gene Synthesis?
- De novo synthesis of complete gene sequences without using a DNA template.
- Allows for codon optimization, sequence modification, and cloning into desired vectors.
📋 Workflow
- Sequence design or optimization
- Fragment assembly and error correction
- Cloning into plasmid/vector
- Sequence verification and delivery
🎯 Applications
- Protein expression and functional studies
- Synthetic biology and pathway engineering
- Vaccine and antibody development
- CRISPR/Cas gene editing templates
⭐ Key Benefits
- Fully customizable gene design
- Codon optimization for target species
- Ready-to-use cloned constructs
- Saves time compared to traditional cloning
Sanger Sequencing
What is Amplicon Sequencing?
- Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
- Ideal for analyzing known sequences with high accuracy and reliability.
📋 Workflow
- Primer design
- PCR amplification of target region
- Purification and Sanger sequencing
- Sequence analysis and comparison
🎯 Applications
- Mutation detection and SNP validation
- Species identification (e.g., COI, 16S)
- Microbial strain analysis
- CRISPR editing verification
- Sequence confirmation in molecular cloning
⭐ Key Benefits
- High accuracy
- Suitable for small sample numbers
- Cost-effective and easy to perform
What is Plasmid Sequencing?
- Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
- Typically performed using universal primers or custom-designed primers.
📋 Workflow
- Plasmid DNA extraction
- Primer selection (e.g., M13, T7, or custom)
- Sanger sequencing reaction
- Sequence analysis and verification
🎯 Applications
- Validate gene construct accuracy
- Confirm insert sequence and orientation
- Detect mutations or splice sites
- Prepare sequences for database submission (e.g., GenBank)
⭐ Key Benefits
- High accuracy with ~600–1000 bp read length
- Suitable for small inserts or full plasmid sequencing
- Fast, easy, and cost-effective
Short-Read Sequencing ( Illumina )
What is Amplicon Sequencing (Short-Read)?
- Uses PCR to amplify specific target regions of DNA.
- Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
- Suitable for analyzing specific genes or markers across many samples.
📋 Workflow
- Primer design (target-specific)
- PCR amplification of target regions
- Library preparation and indexing
- Illumina short-read sequencing
- Data analysis and variant calling
🎯 Applications
- Microbiome profiling (e.g., 16S/ITS)
- Pathogen detection
- Genetic variation and SNP analysis
- Environmental DNA (eDNA) studies
- Cancer hotspot mutation screening
⭐ Key Benefits
- High-throughput and cost-efficient
- Ideal for multiplexing large sample sets
- High sensitivity for low-frequency variants
- Suitable for targeted regions up to ~600 bp
What is Plasmid Sequencing (Short-Read)?
- Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
- Ideal for full plasmid verification or multiplexed plasmid analysis.
📋 Workflow
- Plasmid DNA extraction
- Fragmentation and library preparation
- Illumina short-read sequencing
- Assembly and sequence verification
🎯 Applications
- Full plasmid sequence confirmation
- Detection of point mutations, indels, or rearrangements
- High-throughput screening of plasmid libraries
- QC for plasmid-based gene therapy or vaccine vectors
⭐ Key Benefits
- Ultra-high accuracy and depth
- Suitable for large plasmids and pooled samples
- Allows full plasmid reconstruction
- Scalable and automation-friendly
What is mRNA-seq?
- Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
- Captures a snapshot of active genes in cells or tissues.
📋 Workflow
- RNA extraction
- mRNA enrichment or rRNA depletion
- cDNA synthesis and library preparation
- Illumina sequencing
- Transcript quantification and analysis
🎯 Applications
- Differential gene expression analysis
- Biomarker discovery
- Pathway and functional analysis
- Disease mechanism research (e.g., cancer, infection)
- Drug response and toxicogenomics
⭐ Key Benefits
- High sensitivity and reproducibility
- Suitable for a wide range of samples
- Enables whole-transcriptome analysis
- Scalable for large sample numbers
What is Whole Exome Sequencing (WES)?
- Targets and sequences all protein-coding regions (exons) of the human genome.
- Covers ~1–2% of the genome but contains ~85% of known disease-related variants.
📋 Workflow
- Genomic DNA extraction
- Exome capture/enrichment
- Library preparation and Illumina sequencing
- Variant calling and annotation
🎯 Applications
- Rare disease gene discovery
- Cancer genomics and somatic mutation analysis
- Inherited disease and carrier screening
- Personalized and precision medicine
⭐ Key Benefits
- Focused on clinically relevant regions
- Cost-effective compared to whole-genome sequencing
- High coverage and accuracy
- Scalable for large cohort studies
What is Whole Genome Sequencing (WGS)?
- Sequences the entire human genome, including coding and non-coding regions.
- Provides the most comprehensive view of genetic variation.
📋 Workflow
- Genomic DNA extraction
- Library preparation
- Illumina short-read sequencing
- Read alignment and variant analysis
🎯 Applications
- Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
- Population genetics and evolutionary studies
- Disease gene discovery
- Cancer genomics and precision medicine
⭐ Key Benefits
- Covers the entire genome
- High data quality and depth
- Ideal for discovery-based research
- Supports large-scale studies and biobanks
What is Shotgun Metagenomic Sequencing?
- Randomly sequences all DNA in a sample to profile entire microbial communities.
- Provides insights into both taxonomy and functional potential (genes, pathways).
📋 Workflow
- DNA extraction from environmental or biological samples
- Library preparation
- Illumina short-read sequencing
- Taxonomic classification and functional annotation
🎯 Applications
- Microbiome profiling (gut, soil, marine, etc.)
- Detection of pathogens and antimicrobial resistance genes
- Environmental and industrial microbial studies
- Functional analysis of complex communities
⭐ Key Benefits
- Species- and strain-level resolution
- Simultaneous detection of bacteria, viruses, fungi, and more
- Functional gene profiling (not just "who", but "what they do")
- Culture-independent and unbiased
What is RAD-seq?
- A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
- Efficient for genotyping across the genome without sequencing the whole genome.
📋 Workflow
- Genomic DNA digestion with restriction enzymes
- Adapter ligation and PCR amplification
- Library preparation and Illumina sequencing
- SNP discovery and genotyping
🎯 Applications
- Population genetics and phylogenetics
- Linkage mapping and QTL analysis
- Genomic selection and marker development
- Studies of non-model organisms
⭐ Key Benefits
- Cost-effective genome-wide variant discovery
- Works without a reference genome
- Suitable for large numbers of samples
- High-resolution genotyping for evolutionary studies
Long-Read Sequencing ( Nanopore / PacBio )
Nanopore
What is Long-Read WGS?
- Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
- Enables accurate assembly and detection of complex variants across the entire genome.
📋 Workflow
- High-molecular-weight DNA extraction
- Library preparation (no PCR required)
- Nanopore long-read sequencing
- Read alignment and genome analysis
🎯 Applications
- Structural variant detection (SVs, CNVs, inversions)
- De novo genome assembly
- Disease gene discovery and genome phasing
- Cancer genomics and epigenetic analysis (e.g., methylation)
⭐ Key Benefits
- Ultra-long reads for resolving complex regions
- PCR-free workflow preserves native DNA modifications
- Detects variants missed by short-read methods
- Enables full haplotype phasing and improved assembly
What is Long-Read Plasmid Sequencing?
- Uses Nanopore sequencing to read entire plasmid molecules in a single run.
- Provides complete, circularized plasmid sequences without assembly gaps.
📋 Workflow
- Plasmid DNA extraction (high purity)
- Library preparation (minimal fragmentation)
- Nanopore long-read sequencing
- Circular assembly and variant analysis
🎯 Applications
- Full plasmid verification (including insert, backbone, and junctions)
- Detection of mutations, rearrangements, or contamination
- Characterization of gene therapy or vaccine vectors
- Synthetic biology construct validation
⭐ Key Benefits
- Complete plasmid reconstruction in one read
- High accuracy and structural resolution
- PCR-free workflow preserves native sequence context
- Fast turnaround and scalable for multiple constructs
What is Microbial WGS (Long-Read)?
- Uses Nanopore sequencing to generate complete microbial genomes in single runs.
- Enables accurate assembly of chromosomes, plasmids, and mobile elements.
📋 Workflow
- High-quality microbial DNA extraction
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- De novo assembly and genome annotation
🎯 Applications
- Complete microbial genome reconstruction
- Identification of plasmids, phages, and resistance genes
- Comparative genomics and strain typing
- Pathogen surveillance and outbreak tracing
⭐ Key Benefits
- Resolves repetitive regions and structural variants
- Generates finished, circular genomes
- Culture-independent and fast
- Ideal for clinical, environmental, or industrial strains
What is Long-Read Amplicon Sequencing?
- Uses Nanopore sequencing to read full-length PCR amplicons.
- Enables sequencing of long or complex regions that are difficult for short-read platforms.
📋 Workflow
- Target-specific PCR amplification
- Barcoding and library preparation
- Nanopore long-read sequencing
- Consensus building and variant analysis
🎯 Applications
- Full-length 16S/ITS rRNA gene profiling
- HLA genotyping and immune repertoire analysis
- Detection of structural variants in specific genes
- Pathogen identification and strain-level resolution
⭐ Key Benefits
- Long reads cover entire amplicons in single reads
- No assembly required, less bias
- High-resolution taxonomic and genetic analysis
- Scalable and multiplex-friendly
What is Long-Read Metagenomic Sequencing?
- Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
- Captures complete microbial genomes and resolves complex communities.
📋 Workflow
- DNA extraction from environmental or clinical samples
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- Taxonomic and functional analysis
🎯 Applications
- Microbiome analysis at species or strain level
- Detection of antimicrobial resistance genes and plasmids
- Viral, bacterial, and fungal pathogen identification
- Real-time monitoring of environmental or clinical samples
⭐ Key Benefits
- Full-length reads enable species- and strain-level resolution
- No PCR bias; captures low-abundance or novel organisms
- Enables genome reconstruction from metagenomic data
- Fast and portable sequencing for field applications
PacBio
What is PacBio Amplicon Sequencing?
- Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
- Ideal for complex or repetitive regions, and for distinguishing closely related variants.
📋 Workflow
- Target-specific PCR amplification
- SMRTbell library preparation
- PacBio long-read sequencing (HiFi reads)
- Consensus sequence generation and analysis
🎯 Applications
- Full-length 16S/ITS rRNA profiling
- HLA typing and immune repertoire analysis
- Detection of rare variants and gene isoforms
- Amplicon-based microbial community studies
⭐ Key Benefits
- High accuracy (HiFi reads ≥99.9%)
- Full-length reads without assembly
- Resolves complex or repetitive regions
- Ideal for highly similar sequences or low-frequency variants
Clinical Testing Services/Reagents
What is Pharmacogenetic qPCR Testing?
- Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
- Based on real-time PCR for rapid and reliable genotyping.
📋 Workflow
- Sample collection (e.g., buccal swab or blood)
- DNA extraction
- qPCR amplification with gene-specific probes
- Genotype analysis and interpretation
🎯 Applications
- Personalized medicine and drug response prediction
- Dose adjustment for anticoagulants, antidepressants, etc.
- Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
- Reducing risk of adverse drug reactions
⭐ Key Benefits
- Fast, accurate, and cost-effective
- Compatible with clinical sample types
- Easy-to-use format for clinical labs
- Enables precision treatment decisions
What is TSO500?
- A comprehensive NGS panel for analyzing 500+ cancer-related genes.
- Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.
📋 Workflow
- DNA/RNA extraction from FFPE or fresh samples
- Library preparation and hybrid capture
- Illumina sequencing (NextSeq/NovaSeq)
- Bioinformatic analysis and clinical interpretation
🎯 Applications
- Comprehensive tumor profiling
- Identification of actionable mutations and drug targets
- Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
- Support for precision oncology and immunotherapy decisions
⭐ Key Benefits
- All-in-one assay for solid tumor characterization
- High sensitivity and specificity
- Compatible with clinical sample types (FFPE)
- Enables personalized cancer treatment strategies
What is Hereditary Disease Testing?
- Analyzes inherited genetic variants associated with hereditary disorders.
- Helps assess disease risk, carrier status, and guide family planning.
📋 Workflow
- Sample collection (blood or saliva)
- DNA extraction and library preparation
- NGS or panel-based sequencing
- Variant interpretation and clinical reporting
🎯 Applications
- Screening for inherited cancer syndromes (e.g., BRCA1/2)
- Carrier testing for autosomal recessive disorders
- Genetic diagnosis of rare diseases
- Family risk assessment and genetic counseling
⭐ Key Benefits
- Early detection and prevention
- Supports personalized treatment decisions
- Informative for reproductive planning
- Comprehensive panels for multiple conditions
Sanger Sequencing
What is Amplicon Sequencing?
- Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
- Ideal for analyzing known sequences with high accuracy and reliability.
📋 Workflow
- Primer design
- PCR amplification of target region
- Purification and Sanger sequencing
- Sequence analysis and comparison
🎯 Applications
- Mutation detection and SNP validation
- Species identification (e.g., COI, 16S)
- Microbial strain analysis
- CRISPR editing verification
- Sequence confirmation in molecular cloning
⭐ Key Benefits
- High accuracy
- Suitable for small sample numbers
- Cost-effective and easy to perform
What is Plasmid Sequencing?
- Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
- Typically performed using universal primers or custom-designed primers.
📋 Workflow
- Plasmid DNA extraction
- Primer selection (e.g., M13, T7, or custom)
- Sanger sequencing reaction
- Sequence analysis and verification
🎯 Applications
- Validate gene construct accuracy
- Confirm insert sequence and orientation
- Detect mutations or splice sites
- Prepare sequences for database submission (e.g., GenBank)
⭐ Key Benefits
- High accuracy with ~600–1000 bp read length
- Suitable for small inserts or full plasmid sequencing
- Fast, easy, and cost-effective
Short-Read Sequencing ( Illumina )
What is Amplicon Sequencing (Short-Read)?
- Uses PCR to amplify specific target regions of DNA.
- Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
- Suitable for analyzing specific genes or markers across many samples.
📋 Workflow
- Primer design (target-specific)
- PCR amplification of target regions
- Library preparation and indexing
- Illumina short-read sequencing
- Data analysis and variant calling
🎯 Applications
- Microbiome profiling (e.g., 16S/ITS)
- Pathogen detection
- Genetic variation and SNP analysis
- Environmental DNA (eDNA) studies
- Cancer hotspot mutation screening
⭐ Key Benefits
- High-throughput and cost-efficient
- Ideal for multiplexing large sample sets
- High sensitivity for low-frequency variants
- Suitable for targeted regions up to ~600 bp
What is Plasmid Sequencing (Short-Read)?
- Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
- Ideal for full plasmid verification or multiplexed plasmid analysis.
📋 Workflow
- Plasmid DNA extraction
- Fragmentation and library preparation
- Illumina short-read sequencing
- Assembly and sequence verification
🎯 Applications
- Full plasmid sequence confirmation
- Detection of point mutations, indels, or rearrangements
- High-throughput screening of plasmid libraries
- QC for plasmid-based gene therapy or vaccine vectors
⭐ Key Benefits
- Ultra-high accuracy and depth
- Suitable for large plasmids and pooled samples
- Allows full plasmid reconstruction
- Scalable and automation-friendly
What is Whole Genome Sequencing (WGS)?
- Sequences the entire human genome, including coding and non-coding regions.
- Provides the most comprehensive view of genetic variation.
📋 Workflow
- Genomic DNA extraction
- Library preparation
- Illumina short-read sequencing
- Read alignment and variant analysis
🎯 Applications
- Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
- Population genetics and evolutionary studies
- Disease gene discovery
- Cancer genomics and precision medicine
⭐ Key Benefits
- Covers the entire genome
- High data quality and depth
- Ideal for discovery-based research
- Supports large-scale studies and biobanks
What is Shotgun Metagenomic Sequencing?
- Randomly sequences all DNA in a sample to profile entire microbial communities.
- Provides insights into both taxonomy and functional potential (genes, pathways).
📋 Workflow
- DNA extraction from environmental or biological samples
- Library preparation
- Illumina short-read sequencing
- Taxonomic classification and functional annotation
🎯 Applications
- Microbiome profiling (gut, soil, marine, etc.)
- Detection of pathogens and antimicrobial resistance genes
- Environmental and industrial microbial studies
- Functional analysis of complex communities
⭐ Key Benefits
- Species- and strain-level resolution
- Simultaneous detection of bacteria, viruses, fungi, and more
- Functional gene profiling (not just "who", but "what they do")
- Culture-independent and unbiased
What is RAD-seq?
- A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
- Efficient for genotyping across the genome without sequencing the whole genome.
📋 Workflow
- Genomic DNA digestion with restriction enzymes
- Adapter ligation and PCR amplification
- Library preparation and Illumina sequencing
- SNP discovery and genotyping
🎯 Applications
- Population genetics and phylogenetics
- Linkage mapping and QTL analysis
- Genomic selection and marker development
- Studies of non-model organisms
⭐ Key Benefits
- Cost-effective genome-wide variant discovery
- Works without a reference genome
- Suitable for large numbers of samples
- High-resolution genotyping for evolutionary studies
Long-Read Sequencing ( Nanopore / PacBio )
Nanopore
What is Long-Read WGS?
- Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
- Enables accurate assembly and detection of complex variants across the entire genome.
📋 Workflow
- High-molecular-weight DNA extraction
- Library preparation (no PCR required)
- Nanopore long-read sequencing
- Read alignment and genome analysis
🎯 Applications
- Structural variant detection (SVs, CNVs, inversions)
- De novo genome assembly
- Disease gene discovery and genome phasing
- Cancer genomics and epigenetic analysis (e.g., methylation)
⭐ Key Benefits
- Ultra-long reads for resolving complex regions
- PCR-free workflow preserves native DNA modifications
- Detects variants missed by short-read methods
- Enables full haplotype phasing and improved assembly
What is Long-Read Plasmid Sequencing?
- Uses Nanopore sequencing to read entire plasmid molecules in a single run.
- Provides complete, circularized plasmid sequences without assembly gaps.
📋 Workflow
- Plasmid DNA extraction (high purity)
- Library preparation (minimal fragmentation)
- Nanopore long-read sequencing
- Circular assembly and variant analysis
🎯 Applications
- Full plasmid verification (including insert, backbone, and junctions)
- Detection of mutations, rearrangements, or contamination
- Characterization of gene therapy or vaccine vectors
- Synthetic biology construct validation
⭐ Key Benefits
- Complete plasmid reconstruction in one read
- High accuracy and structural resolution
- PCR-free workflow preserves native sequence context
- Fast turnaround and scalable for multiple constructs
What is Microbial WGS (Long-Read)?
- Uses Nanopore sequencing to generate complete microbial genomes in single runs.
- Enables accurate assembly of chromosomes, plasmids, and mobile elements.
📋 Workflow
- High-quality microbial DNA extraction
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- De novo assembly and genome annotation
🎯 Applications
- Complete microbial genome reconstruction
- Identification of plasmids, phages, and resistance genes
- Comparative genomics and strain typing
- Pathogen surveillance and outbreak tracing
⭐ Key Benefits
- Resolves repetitive regions and structural variants
- Generates finished, circular genomes
- Culture-independent and fast
- Ideal for clinical, environmental, or industrial strains
What is Long-Read Amplicon Sequencing?
- Uses Nanopore sequencing to read full-length PCR amplicons.
- Enables sequencing of long or complex regions that are difficult for short-read platforms.
📋 Workflow
- Target-specific PCR amplification
- Barcoding and library preparation
- Nanopore long-read sequencing
- Consensus building and variant analysis
🎯 Applications
- Full-length 16S/ITS rRNA gene profiling
- HLA genotyping and immune repertoire analysis
- Detection of structural variants in specific genes
- Pathogen identification and strain-level resolution
⭐ Key Benefits
- Long reads cover entire amplicons in single reads
- No assembly required, less bias
- High-resolution taxonomic and genetic analysis
- Scalable and multiplex-friendly
What is Long-Read Metagenomic Sequencing?
- Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
- Captures complete microbial genomes and resolves complex communities.
📋 Workflow
- DNA extraction from environmental or clinical samples
- Library preparation (PCR-free)
- Nanopore long-read sequencing
- Taxonomic and functional analysis
🎯 Applications
- Microbiome analysis at species or strain level
- Detection of antimicrobial resistance genes and plasmids
- Viral, bacterial, and fungal pathogen identification
- Real-time monitoring of environmental or clinical samples
⭐ Key Benefits
- Full-length reads enable species- and strain-level resolution
- No PCR bias; captures low-abundance or novel organisms
- Enables genome reconstruction from metagenomic data
- Fast and portable sequencing for field applications
PacBio
What is PacBio Amplicon Sequencing?
- Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
- Ideal for complex or repetitive regions, and for distinguishing closely related variants.
📋 Workflow
- Target-specific PCR amplification
- SMRTbell library preparation
- PacBio long-read sequencing (HiFi reads)
- Consensus sequence generation and analysis
🎯 Applications
- Full-length 16S/ITS rRNA profiling
- HLA typing and immune repertoire analysis
- Detection of rare variants and gene isoforms
- Amplicon-based microbial community studies
⭐ Key Benefits
- High accuracy (HiFi reads ≥99.9%)
- Full-length reads without assembly
- Resolves complex or repetitive regions
- Ideal for highly similar sequences or low-frequency variants
Clinical Testing Services/Reagents
What is Pharmacogenetic qPCR Testing?
- Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
- Based on real-time PCR for rapid and reliable genotyping.
📋 Workflow
- Sample collection (e.g., buccal swab or blood)
- DNA extraction
- qPCR amplification with gene-specific probes
- Genotype analysis and interpretation
🎯 Applications
- Personalized medicine and drug response prediction
- Dose adjustment for anticoagulants, antidepressants, etc.
- Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
- Reducing risk of adverse drug reactions
⭐ Key Benefits
- Fast, accurate, and cost-effective
- Compatible with clinical sample types
- Easy-to-use format for clinical labs
- Enables precision treatment decisions
What is TSO500?
- A comprehensive NGS panel for analyzing 500+ cancer-related genes.
- Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.
📋 Workflow
- DNA/RNA extraction from FFPE or fresh samples
- Library preparation and hybrid capture
- Illumina sequencing (NextSeq/NovaSeq)
- Bioinformatic analysis and clinical interpretation
🎯 Applications
- Comprehensive tumor profiling
- Identification of actionable mutations and drug targets
- Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
- Support for precision oncology and immunotherapy decisions
⭐ Key Benefits
- All-in-one assay for solid tumor characterization
- High sensitivity and specificity
- Compatible with clinical sample types (FFPE)
- Enables personalized cancer treatment strategies
What is Hereditary Disease Testing?
- Analyzes inherited genetic variants associated with hereditary disorders.
- Helps assess disease risk, carrier status, and guide family planning.
📋 Workflow
- Sample collection (blood or saliva)
- DNA extraction and library preparation
- NGS or panel-based sequencing
- Variant interpretation and clinical reporting
🎯 Applications
- Screening for inherited cancer syndromes (e.g., BRCA1/2)
- Carrier testing for autosomal recessive disorders
- Genetic diagnosis of rare diseases
- Family risk assessment and genetic counseling
⭐ Key Benefits
- Early detection and prevention
- Supports personalized treatment decisions
- Informative for reproductive planning
- Comprehensive panels for multiple conditions
Others
What is Oligo Synthesis?
- Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
- Customized for specific research, diagnostic, or therapeutic purposes.
📋 Workflow
- Sequence design and order placement
- Automated phosphoramidite synthesis
- Deprotection and purification
- Quality control (e.g., MALDI-TOF, OD260)
🎯 Applications
- PCR, qPCR, and RT-PCR primers
- Probes for molecular diagnostics
- Gene editing (e.g., CRISPR guide RNAs)
- siRNA, antisense, or aptamer research
⭐ Key Benefits
- High purity and customization options
- Fast turnaround and scalable production
- Modified bases, labels, and delivery formats available
- Reliable support for research and clinical needs
What is Whole Gene Synthesis?
- De novo synthesis of complete gene sequences without using a DNA template.
- Allows for codon optimization, sequence modification, and cloning into desired vectors.
📋 Workflow
- Sequence design or optimization
- Fragment assembly and error correction
- Cloning into plasmid/vector
- Sequence verification and delivery
🎯 Applications
- Protein expression and functional studies
- Synthetic biology and pathway engineering
- Vaccine and antibody development
- CRISPR/Cas gene editing templates
⭐ Key Benefits
- Fully customizable gene design
- Codon optimization for target species
- Ready-to-use cloned constructs
- Saves time compared to traditional cloning
Short-Read Sequencing ( Illumina )
What is mRNA-seq?
- Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
- Captures a snapshot of active genes in cells or tissues.
📋 Workflow
- RNA extraction
- mRNA enrichment or rRNA depletion
- cDNA synthesis and library preparation
- Illumina sequencing
- Transcript quantification and analysis
🎯 Applications
- Differential gene expression analysis
- Biomarker discovery
- Pathway and functional analysis
- Disease mechanism research (e.g., cancer, infection)
- Drug response and toxicogenomics
⭐ Key Benefits
- High sensitivity and reproducibility
- Suitable for a wide range of samples
- Enables whole-transcriptome analysis
- Scalable for large sample numbers
What is Whole Exome Sequencing (WES)?
- Targets and sequences all protein-coding regions (exons) of the human genome.
- Covers ~1–2% of the genome but contains ~85% of known disease-related variants.
📋 Workflow
- Genomic DNA extraction
- Exome capture/enrichment
- Library preparation and Illumina sequencing
- Variant calling and annotation
🎯 Applications
- Rare disease gene discovery
- Cancer genomics and somatic mutation analysis
- Inherited disease and carrier screening
- Personalized and precision medicine
⭐ Key Benefits
- Focused on clinically relevant regions
- Cost-effective compared to whole-genome sequencing
- High coverage and accuracy
- Scalable for large cohort studies
Others
What is Oligo Synthesis?
- Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
- Customized for specific research, diagnostic, or therapeutic purposes.
📋 Workflow
- Sequence design and order placement
- Automated phosphoramidite synthesis
- Deprotection and purification
- Quality control (e.g., MALDI-TOF, OD260)
🎯 Applications
- PCR, qPCR, and RT-PCR primers
- Probes for molecular diagnostics
- Gene editing (e.g., CRISPR guide RNAs)
- siRNA, antisense, or aptamer research
⭐ Key Benefits
- High purity and customization options
- Fast turnaround and scalable production
- Modified bases, labels, and delivery formats available
- Reliable support for research and clinical needs
What is Whole Gene Synthesis?
- De novo synthesis of complete gene sequences without using a DNA template.
- Allows for codon optimization, sequence modification, and cloning into desired vectors.
📋 Workflow
- Sequence design or optimization
- Fragment assembly and error correction
- Cloning into plasmid/vector
- Sequence verification and delivery
🎯 Applications
- Protein expression and functional studies
- Synthetic biology and pathway engineering
- Vaccine and antibody development
- CRISPR/Cas gene editing templates
⭐ Key Benefits
- Fully customizable gene design
- Codon optimization for target species
- Ready-to-use cloned constructs
- Saves time compared to traditional cloning
關於艾達特
關於艾達特
我們以「艾達特」命名並成立公司,概念取自於定序過程中Adaptor(轉接子)的作用, 期許我們能在生物與資訊領域中扮演承接與轉化的角色,成為學術界與產業界在樣品庫前端製備、 後端數據分析的最佳合作夥伴,讓彼此在快速變遷的時代中,能以適存者 (adaptor) 之姿克服挑戰,共創無限的可能。

發展目標
艾達特致力於建構「客戶導向之基因體技術整合服務平台」,自前端樣品製備建庫至後端數據處理分析提供系列化服務,旨在開啟新型態基因體技術於臺灣之廣泛應用。本公司現透過與高雄醫學大學產學合作,強化基因體科學以及產業應用鏈結,藉以提高服務平台各環節之整合性與完整度,進而依照客戶之學術亦或產業需求,為其量身打造客製化分析流程,跳脫一體適用的業界訴求,以「理解需求,購買所求」模式,精準突破當前生物科學市場困境。
客製化諮詢服務
提供基因體技術諮詢,依照您希望應用的技術項目進行應用說明,
幫助您了解即將進行的服務品項與分析結果,
客製化諮詢服務
提供基因體技術諮詢,依照您希望應用的技術項目進行應用說明,
幫助您了解即將進行的服務品項與分析結果,
銜接您的計畫、
目標與本公司所提供的服務品項,並客製化您所享有的基因體服務。
技術應用諮詢
以本公司所提供之RAD-seq、RNA 基因體技術服務品項為主,提供實驗前討論諮詢。
生物資訊諮詢
依照您所取得的生物資訊數據,進行分析前諮詢,確保您所使用的基因體服務符合您的需求。
客製化數據分析
本公司同時提供您DNA、RNA的客製化分析,可依照您提供的參考文獻進行分析,分析如親緣關係樹建立、遺傳結構分析等。
艾達特團隊介紹
艾達特團隊介紹

邱奕凡
執行長生物資訊部總監

李菡勻
營運長技術研發部總監

萬郁岑
研發工程師生物資訊部門

李沂晏
數據分析師生物資訊部門
聯絡我們
聯絡我們
地址
80708高雄市三民區民族一路80號B10辦公室2樓之1(B10)
連絡電話
07-9769339
聯絡信箱
生物資訊部 yfchiu@adaptor-genosci.com
營運管理部 hyli@adaptor-genosci.com