我們是連結生物與資訊的轉接子

不是實驗的起點，也不是答案的終點，但我們讓兩者連成一線。

了解更多關於艾達特

成立年

經手樣品數

經手物種種類

國內外送案單位

技術應用諮詢

生物資訊諮詢

客製化數據分析

ABOUT OUR COMPANY

關於艾達特

艾達特以定序過程中的 Adaptor（轉接子）為靈感命名，象徵我們在生物與資訊之間承接與轉化的角色。我們致力於從前端樣品建庫到後端數據分析，提供完整而專業的基因體技術服務，成為學術與產業的可信賴夥伴。在快速變動的時代，我們秉持適存者 (Adaptor) 的精神，協助合作夥伴突破挑戰，開創更多應用與可能。

Development Goals

發展目標

艾達特致力於建構以客戶需求為核心的基因體技術整合服務平台，推動基因體科技在臺灣及國際的廣泛應用。我們將持續發展：

全流程整合：從樣品製備到數據分析，提供一站式解決方案。
技術創新：持續引入新世代定序與分析技術，提升服務精準度與效率。
國際合作：承接海外專案，並與菲律賓簽署代理，逐步拓展國際市場。
客製化分析：跳脫傳統「一體適用」模式，依據不同產業與研究需求，打造精準、彈性的分析流程。

2025.01 與菲律賓 Manila HealthTek 簽訂代理合約

客製化諮詢服務

技術應用諮詢

以本公司所提供之RAD-seq、RNA 基因體技術服務品項為主，提供實驗前討論諮詢。

生物資訊諮詢

依照您所取得的生物資訊數據，進行分析前諮詢，確保您所使用的基因體服務符合您的需求。

客製化數據分析

本公司同時提供您DNA、RNA的客製化分析，可依照您提供的參考文獻進行分析，分析如親緣關係樹建立、遺傳結構分析等。

技術服務

艾達特提供全方位基因體服務，涵蓋 RAD-seq、RNA 定序、總體基因體（宏基因體）等技術
使用 Illumina 與 Nanopore 平台，從樣本建庫、定序到生物資訊分析，協助推動學術研究、農業改良與產業應用。

次世代定序( Illumina )

What is Amplicon Sequencing (Short-Read)?

Uses PCR to amplify specific target regions of DNA.
Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

Primer design (target-specific)
PCR amplification of target regions
Library preparation and indexing
Illumina short-read sequencing
Data analysis and variant calling

🎯 Applications

Microbiome profiling (e.g., 16S/ITS)
Pathogen detection
Genetic variation and SNP analysis
Environmental DNA (eDNA) studies
Cancer hotspot mutation screening

⭐ Key Benefits

High-throughput and cost-efficient
Ideal for multiplexing large sample sets
High sensitivity for low-frequency variants
Suitable for targeted regions up to ~600 bp

What is mRNA-seq?

Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
Captures a snapshot of active genes in cells or tissues.

📋 Workflow

RNA extraction
mRNA enrichment or rRNA depletion
cDNA synthesis and library preparation
Illumina sequencing
Transcript quantification and analysis

🎯 Applications

Differential gene expression analysis
Biomarker discovery
Pathway and functional analysis
Disease mechanism research (e.g., cancer, infection)
Drug response and toxicogenomics

⭐ Key Benefits

High sensitivity and reproducibility
Suitable for a wide range of samples
Enables whole-transcriptome analysis
Scalable for large sample numbers

What is Shotgun Metagenomic Sequencing?

Randomly sequences all DNA in a sample to profile entire microbial communities.
Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

DNA extraction from environmental or biological samples
Library preparation
Illumina short-read sequencing
Taxonomic classification and functional annotation

🎯 Applications

Microbiome profiling (gut, soil, marine, etc.)
Detection of pathogens and antimicrobial resistance genes
Environmental and industrial microbial studies
Functional analysis of complex communities

⭐ Key Benefits

Species- and strain-level resolution
Simultaneous detection of bacteria, viruses, fungi, and more
Functional gene profiling (not just "who", but "what they do")
Culture-independent and unbiased

RAD-seq限制酶點位核酸定序

RAD-seq：簡化基因體，從建庫到分析

限制酶點位核酸定序服務（RAD-seq，Restriction-site Associated DNA Sequencing），以限制酶簡化基因體資訊，提升有效數據比例，可同時處理大量族群樣本，降低研究負擔。RAD-seq 可應用於無參考序列之目標物種的族群遺傳變異、定義個體間遺傳差異、族群親緣地理關係追溯等生物演化研究；同時適用於單核苷酸多態性（SNP，Single Nucleotide Polymorphism）分子標誌建立，可有效應用於農林漁牧等相關產業。

RAD-seq 技術核心

RAD-seq 是結合了限制酶處理和高通量定序優勢的簡化基因體學技術，用於檢測與分析基因體間的遺傳變異。
以限制酶切位和片段長度為篩選條件，使 RAD-seq 無需對整個基因體進行定序，降低定序深度需求，同時提高樣品間相似片段的有效序列定序比例。
RAD-seq 具有多樣品混合定序的特性，使用 DNA 標籤技術（DNA barcoding），可同時研究大量樣本，快速處理個體/族群間的基因體變異，獲得 SNPs 資訊。
技術廣泛應用於研究自然種群中的遺傳多樣性、物種分化、突變和進化，以及全基因關聯分析（GWAS）和數量性狀基因座定位（QTL mapping）等研究領域。

RAD-seq 建庫介紹

Digestion & Ligation:

利用限制酶將 DNA 切割為較小的片段，針對其切位將 DNA 片段標記上特定標籤序列，每個樣本都有唯一的標記序列，用以區分來自不同樣本的片段。

Pooling:

將來自不同樣本的 DNA 片段混合在一起，形成 DNA 樣品庫。

Size Selection:

經由 Size Selection 保留適合上機定序的片段長度，同時去除過短的片段。

PCR:

使用 PCR 反應增殖 DNA 片段，同時完成 DNA 樣品庫的製備，準備進行 NovaSeq 高通量定序。

Sequencing:

完成定序後，對序列進行比對、組裝和分析，獲取樣本中基因體變異的資訊，得到 SNPs 資訊。

標準服務

您只需要提供DNA樣品，即可享有樣品建庫、定序，以及生物資訊組裝服務。組裝後的數據可直接供後續各項分析使用，免除研究人員實驗前置作業、數據組裝與處理格式問題所耗時間及設備成本。

進階/客製化分析服務

艾達特提供 RAD-seq 組裝後的衍生分析，可輸出符合指定期刊格式之科學圖表。依照需求客製化製圖，強調分析成果亮點，並提供兩次修改與重分析服務，確保分析內容符合研究探討主軸，可提供親緣關係樹分析（Phylogenetic Tree）、族群遺傳結構分析（Population genetic structure）、主成分分析（PCA/DAPC）等，除上列進階分析項目，我們也提供客製化分析服務，可依照您提供的參考文獻進行分析評估，如基因隔離地圖（Dispersal map of resistance）、遺傳分化係數分析（Fixation index analysis）、全基因組關聯分析（GWAS）或數量性狀基因座定位（QTL mapping）等。

技術諮詢/樣品測試服務

在您決定使用艾達特的服務之前，您可以聯繫我們，讓我們為您進行 RAD-seq 的基礎介紹以及研究、應用方向的討論，確保艾達特提供的服務能契合您的期望，同時提供給您樣品測試的服務，透過少量的樣品進行酵素適用性測試，確認您的目標樣品所適合的製備方式。

第三代定序( Nanopore )

What is Long-Read WGS?

Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

High-molecular-weight DNA extraction
Library preparation (no PCR required)
Nanopore long-read sequencing
Read alignment and genome analysis

🎯 Applications

Structural variant detection (SVs, CNVs, inversions)
De novo genome assembly
Disease gene discovery and genome phasing
Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

Ultra-long reads for resolving complex regions
PCR-free workflow preserves native DNA modifications
Detects variants missed by short-read methods
Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

Uses Nanopore sequencing to read entire plasmid molecules in a single run.
Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

Plasmid DNA extraction (high purity)
Library preparation (minimal fragmentation)
Nanopore long-read sequencing
Circular assembly and variant analysis

🎯 Applications

Full plasmid verification (including insert, backbone, and junctions)
Detection of mutations, rearrangements, or contamination
Characterization of gene therapy or vaccine vectors
Synthetic biology construct validation

⭐ Key Benefits

Complete plasmid reconstruction in one read
High accuracy and structural resolution
PCR-free workflow preserves native sequence context
Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

Uses Nanopore sequencing to generate complete microbial genomes in single runs.
Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

High-quality microbial DNA extraction
Library preparation (PCR-free)
Nanopore long-read sequencing
De novo assembly and genome annotation

🎯 Applications

Complete microbial genome reconstruction
Identification of plasmids, phages, and resistance genes
Comparative genomics and strain typing
Pathogen surveillance and outbreak tracing

⭐ Key Benefits

Resolves repetitive regions and structural variants
Generates finished, circular genomes
Culture-independent and fast
Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

Uses Nanopore sequencing to read full-length PCR amplicons.
Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

Target-specific PCR amplification
Barcoding and library preparation
Nanopore long-read sequencing
Consensus building and variant analysis

🎯 Applications

Full-length 16S/ITS rRNA gene profiling
HLA genotyping and immune repertoire analysis
Detection of structural variants in specific genes
Pathogen identification and strain-level resolution

⭐ Key Benefits

Long reads cover entire amplicons in single reads
No assembly required, less bias
High-resolution taxonomic and genetic analysis
Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
Captures complete microbial genomes and resolves complex communities.

📋 Workflow

DNA extraction from environmental or clinical samples
Library preparation (PCR-free)
Nanopore long-read sequencing
Taxonomic and functional analysis

🎯 Applications

Microbiome analysis at species or strain level
Detection of antimicrobial resistance genes and plasmids
Viral, bacterial, and fungal pathogen identification
Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

Full-length reads enable species- and strain-level resolution
No PCR bias; captures low-abundance or novel organisms
Enables genome reconstruction from metagenomic data
Fast and portable sequencing for field applications