Genome technology integration service platform




2025.01.15 Signed an agency contract with Manila HealthTek in the Philippines

Year of Establishment

Number of Samples Handled

Species Types Handled

Domestic and International Clients


Technical

Technical


Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning

Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions


Sanger Sequencing

What is Amplicon Sequencing?

  • Uses specific primers to amplify target DNA regions via PCR, followed by Sanger sequencing.
  • Ideal for analyzing known sequences with high accuracy and reliability.

📋 Workflow

  1. Primer design
  2. PCR amplification of target region
  3. Purification and Sanger sequencing
  4. Sequence analysis and comparison

🎯 Applications

  • Mutation detection and SNP validation
  • Species identification (e.g., COI, 16S)
  • Microbial strain analysis
  • CRISPR editing verification
  • Sequence confirmation in molecular cloning

⭐ Key Benefits

  • High accuracy
  • Suitable for small sample numbers
  • Cost-effective and easy to perform

What is Plasmid Sequencing?

  • Uses Sanger sequencing to read part or all of a plasmid DNA sequence.
  • Typically performed using universal primers or custom-designed primers.

📋 Workflow

  1. Plasmid DNA extraction
  2. Primer selection (e.g., M13, T7, or custom)
  3. Sanger sequencing reaction
  4. Sequence analysis and verification

🎯 Applications

  • Validate gene construct accuracy
  • Confirm insert sequence and orientation
  • Detect mutations or splice sites
  • Prepare sequences for database submission (e.g., GenBank)

⭐ Key Benefits

  • High accuracy with ~600–1000 bp read length
  • Suitable for small inserts or full plasmid sequencing
  • Fast, easy, and cost-effective

Short-Read Sequencing ( Illumina )

What is Amplicon Sequencing (Short-Read)?

  • Uses PCR to amplify specific target regions of DNA.
  • Sequencing is performed using Illumina’s short-read platforms (e.g., MiSeq).
  • Suitable for analyzing specific genes or markers across many samples.

📋 Workflow

  1. Primer design (target-specific)
  2. PCR amplification of target regions
  3. Library preparation and indexing
  4. Illumina short-read sequencing
  5. Data analysis and variant calling

🎯 Applications

  • Microbiome profiling (e.g., 16S/ITS)
  • Pathogen detection
  • Genetic variation and SNP analysis
  • Environmental DNA (eDNA) studies
  • Cancer hotspot mutation screening

⭐ Key Benefits

  • High-throughput and cost-efficient
  • Ideal for multiplexing large sample sets
  • High sensitivity for low-frequency variants
  • Suitable for targeted regions up to ~600 bp

What is Plasmid Sequencing (Short-Read)?

  • Uses Illumina sequencing to obtain high-coverage, high-accuracy reads of plasmid DNA.
  • Ideal for full plasmid verification or multiplexed plasmid analysis.

📋 Workflow

  1. Plasmid DNA extraction
  2. Fragmentation and library preparation
  3. Illumina short-read sequencing
  4. Assembly and sequence verification

🎯 Applications

  • Full plasmid sequence confirmation
  • Detection of point mutations, indels, or rearrangements
  • High-throughput screening of plasmid libraries
  • QC for plasmid-based gene therapy or vaccine vectors

⭐ Key Benefits

  • Ultra-high accuracy and depth
  • Suitable for large plasmids and pooled samples
  • Allows full plasmid reconstruction
  • Scalable and automation-friendly

What is Whole Genome Sequencing (WGS)?

  • Sequences the entire human genome, including coding and non-coding regions.
  • Provides the most comprehensive view of genetic variation.

📋 Workflow

  1. Genomic DNA extraction
  2. Library preparation
  3. Illumina short-read sequencing
  4. Read alignment and variant analysis

🎯 Applications

  • Comprehensive mutation detection (SNPs, indels, CNVs, SVs)
  • Population genetics and evolutionary studies
  • Disease gene discovery
  • Cancer genomics and precision medicine

⭐ Key Benefits

  • Covers the entire genome
  • High data quality and depth
  • Ideal for discovery-based research
  • Supports large-scale studies and biobanks

What is Shotgun Metagenomic Sequencing?

  • Randomly sequences all DNA in a sample to profile entire microbial communities.
  • Provides insights into both taxonomy and functional potential (genes, pathways).

📋 Workflow

  1. DNA extraction from environmental or biological samples
  2. Library preparation
  3. Illumina short-read sequencing
  4. Taxonomic classification and functional annotation

🎯 Applications

  • Microbiome profiling (gut, soil, marine, etc.)
  • Detection of pathogens and antimicrobial resistance genes
  • Environmental and industrial microbial studies
  • Functional analysis of complex communities

⭐ Key Benefits

  • Species- and strain-level resolution
  • Simultaneous detection of bacteria, viruses, fungi, and more
  • Functional gene profiling (not just "who", but "what they do")
  • Culture-independent and unbiased

What is RAD-seq?

  • A reduced-representation sequencing method that targets DNA regions near restriction enzyme cut sites.
  • Efficient for genotyping across the genome without sequencing the whole genome.

📋 Workflow

  1. Genomic DNA digestion with restriction enzymes
  2. Adapter ligation and PCR amplification
  3. Library preparation and Illumina sequencing
  4. SNP discovery and genotyping

🎯 Applications

  • Population genetics and phylogenetics
  • Linkage mapping and QTL analysis
  • Genomic selection and marker development
  • Studies of non-model organisms

⭐ Key Benefits

  • Cost-effective genome-wide variant discovery
  • Works without a reference genome
  • Suitable for large numbers of samples
  • High-resolution genotyping for evolutionary studies

Long-Read Sequencing ( Nanopore / PacBio )


Nanopore

What is Long-Read WGS?

  • Uses Nanopore sequencing to read very long DNA fragments, capturing full structural information.
  • Enables accurate assembly and detection of complex variants across the entire genome.

📋 Workflow

  1. High-molecular-weight DNA extraction
  2. Library preparation (no PCR required)
  3. Nanopore long-read sequencing
  4. Read alignment and genome analysis

🎯 Applications

  • Structural variant detection (SVs, CNVs, inversions)
  • De novo genome assembly
  • Disease gene discovery and genome phasing
  • Cancer genomics and epigenetic analysis (e.g., methylation)

⭐ Key Benefits

  • Ultra-long reads for resolving complex regions
  • PCR-free workflow preserves native DNA modifications
  • Detects variants missed by short-read methods
  • Enables full haplotype phasing and improved assembly

What is Long-Read Plasmid Sequencing?

  • Uses Nanopore sequencing to read entire plasmid molecules in a single run.
  • Provides complete, circularized plasmid sequences without assembly gaps.

📋 Workflow

  1. Plasmid DNA extraction (high purity)
  2. Library preparation (minimal fragmentation)
  3. Nanopore long-read sequencing
  4. Circular assembly and variant analysis

🎯 Applications

  • Full plasmid verification (including insert, backbone, and junctions)
  • Detection of mutations, rearrangements, or contamination
  • Characterization of gene therapy or vaccine vectors
  • Synthetic biology construct validation

⭐ Key Benefits

  • Complete plasmid reconstruction in one read
  • High accuracy and structural resolution
  • PCR-free workflow preserves native sequence context
  • Fast turnaround and scalable for multiple constructs

What is Microbial WGS (Long-Read)?

  • Uses Nanopore sequencing to generate complete microbial genomes in single runs.
  • Enables accurate assembly of chromosomes, plasmids, and mobile elements.

📋 Workflow

  1. High-quality microbial DNA extraction
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. De novo assembly and genome annotation

🎯 Applications

  • Complete microbial genome reconstruction
  • Identification of plasmids, phages, and resistance genes
  • Comparative genomics and strain typing
  • Pathogen surveillance and outbreak tracing

⭐ Key Benefits

  • Resolves repetitive regions and structural variants
  • Generates finished, circular genomes
  • Culture-independent and fast
  • Ideal for clinical, environmental, or industrial strains

What is Long-Read Amplicon Sequencing?

  • Uses Nanopore sequencing to read full-length PCR amplicons.
  • Enables sequencing of long or complex regions that are difficult for short-read platforms.

📋 Workflow

  1. Target-specific PCR amplification
  2. Barcoding and library preparation
  3. Nanopore long-read sequencing
  4. Consensus building and variant analysis

🎯 Applications

  • Full-length 16S/ITS rRNA gene profiling
  • HLA genotyping and immune repertoire analysis
  • Detection of structural variants in specific genes
  • Pathogen identification and strain-level resolution

⭐ Key Benefits

  • Long reads cover entire amplicons in single reads
  • No assembly required, less bias
  • High-resolution taxonomic and genetic analysis
  • Scalable and multiplex-friendly

What is Long-Read Metagenomic Sequencing?

  • Uses Nanopore sequencing to directly sequence all DNA in a sample without amplification.
  • Captures complete microbial genomes and resolves complex communities.

📋 Workflow

  1. DNA extraction from environmental or clinical samples
  2. Library preparation (PCR-free)
  3. Nanopore long-read sequencing
  4. Taxonomic and functional analysis

🎯 Applications

  • Microbiome analysis at species or strain level
  • Detection of antimicrobial resistance genes and plasmids
  • Viral, bacterial, and fungal pathogen identification
  • Real-time monitoring of environmental or clinical samples

⭐ Key Benefits

  • Full-length reads enable species- and strain-level resolution
  • No PCR bias; captures low-abundance or novel organisms
  • Enables genome reconstruction from metagenomic data
  • Fast and portable sequencing for field applications

PacBio

What is PacBio Amplicon Sequencing?

  • Uses PacBio’s HiFi long reads to sequence full-length amplicons with high accuracy.
  • Ideal for complex or repetitive regions, and for distinguishing closely related variants.

📋 Workflow

  1. Target-specific PCR amplification
  2. SMRTbell library preparation
  3. PacBio long-read sequencing (HiFi reads)
  4. Consensus sequence generation and analysis

🎯 Applications

  • Full-length 16S/ITS rRNA profiling
  • HLA typing and immune repertoire analysis
  • Detection of rare variants and gene isoforms
  • Amplicon-based microbial community studies

⭐ Key Benefits

  • High accuracy (HiFi reads ≥99.9%)
  • Full-length reads without assembly
  • Resolves complex or repetitive regions
  • Ideal for highly similar sequences or low-frequency variants

Clinical Testing Services/Reagents

What is Pharmacogenetic qPCR Testing?

  • Detects genetic variants that affect drug metabolism, efficacy, or risk of adverse reactions.
  • Based on real-time PCR for rapid and reliable genotyping.

📋 Workflow

  1. Sample collection (e.g., buccal swab or blood)
  2. DNA extraction
  3. qPCR amplification with gene-specific probes
  4. Genotype analysis and interpretation

🎯 Applications

  • Personalized medicine and drug response prediction
  • Dose adjustment for anticoagulants, antidepressants, etc.
  • Identifying poor/rapid metabolizers (e.g., CYP2D6, CYP2C19)
  • Reducing risk of adverse drug reactions

⭐ Key Benefits

  • Fast, accurate, and cost-effective
  • Compatible with clinical sample types
  • Easy-to-use format for clinical labs
  • Enables precision treatment decisions

What is TSO500?

  • A comprehensive NGS panel for analyzing 500+ cancer-related genes.
  • Detects multiple variant types including SNVs, indels, CNVs, fusions, and biomarkers like TMB & MSI.

📋 Workflow

  1. DNA/RNA extraction from FFPE or fresh samples
  2. Library preparation and hybrid capture
  3. Illumina sequencing (NextSeq/NovaSeq)
  4. Bioinformatic analysis and clinical interpretation

🎯 Applications

  • Comprehensive tumor profiling
  • Identification of actionable mutations and drug targets
  • Tumor mutational burden (TMB) and microsatellite instability (MSI) analysis
  • Support for precision oncology and immunotherapy decisions

⭐ Key Benefits

  • All-in-one assay for solid tumor characterization
  • High sensitivity and specificity
  • Compatible with clinical sample types (FFPE)
  • Enables personalized cancer treatment strategies

What is Hereditary Disease Testing?

  • Analyzes inherited genetic variants associated with hereditary disorders.
  • Helps assess disease risk, carrier status, and guide family planning.

📋 Workflow

  1. Sample collection (blood or saliva)
  2. DNA extraction and library preparation
  3. NGS or panel-based sequencing
  4. Variant interpretation and clinical reporting

🎯 Applications

  • Screening for inherited cancer syndromes (e.g., BRCA1/2)
  • Carrier testing for autosomal recessive disorders
  • Genetic diagnosis of rare diseases
  • Family risk assessment and genetic counseling

⭐ Key Benefits

  • Early detection and prevention
  • Supports personalized treatment decisions
  • Informative for reproductive planning
  • Comprehensive panels for multiple conditions

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning

Short-Read Sequencing ( Illumina )

What is mRNA-seq?

  • Uses Illumina sequencing to profile gene expression by sequencing messenger RNA (mRNA).
  • Captures a snapshot of active genes in cells or tissues.

📋 Workflow

  1. RNA extraction
  2. mRNA enrichment or rRNA depletion
  3. cDNA synthesis and library preparation
  4. Illumina sequencing
  5. Transcript quantification and analysis

🎯 Applications

  • Differential gene expression analysis
  • Biomarker discovery
  • Pathway and functional analysis
  • Disease mechanism research (e.g., cancer, infection)
  • Drug response and toxicogenomics

⭐ Key Benefits

  • High sensitivity and reproducibility
  • Suitable for a wide range of samples
  • Enables whole-transcriptome analysis
  • Scalable for large sample numbers

What is Whole Exome Sequencing (WES)?

  • Targets and sequences all protein-coding regions (exons) of the human genome.
  • Covers ~1–2% of the genome but contains ~85% of known disease-related variants.

📋 Workflow

  1. Genomic DNA extraction
  2. Exome capture/enrichment
  3. Library preparation and Illumina sequencing
  4. Variant calling and annotation

🎯 Applications

  • Rare disease gene discovery
  • Cancer genomics and somatic mutation analysis
  • Inherited disease and carrier screening
  • Personalized and precision medicine

⭐ Key Benefits

  • Focused on clinically relevant regions
  • Cost-effective compared to whole-genome sequencing
  • High coverage and accuracy
  • Scalable for large cohort studies

Others

What is Oligo Synthesis?

  • Chemical synthesis of short, single- or double-stranded DNA or RNA sequences.
  • Customized for specific research, diagnostic, or therapeutic purposes.

📋 Workflow

  1. Sequence design and order placement
  2. Automated phosphoramidite synthesis
  3. Deprotection and purification
  4. Quality control (e.g., MALDI-TOF, OD260)

🎯 Applications

  • PCR, qPCR, and RT-PCR primers
  • Probes for molecular diagnostics
  • Gene editing (e.g., CRISPR guide RNAs)
  • siRNA, antisense, or aptamer research

⭐ Key Benefits

  • High purity and customization options
  • Fast turnaround and scalable production
  • Modified bases, labels, and delivery formats available
  • Reliable support for research and clinical needs

What is Whole Gene Synthesis?

  • De novo synthesis of complete gene sequences without using a DNA template.
  • Allows for codon optimization, sequence modification, and cloning into desired vectors.

📋 Workflow

  1. Sequence design or optimization
  2. Fragment assembly and error correction
  3. Cloning into plasmid/vector
  4. Sequence verification and delivery

🎯 Applications

  • Protein expression and functional studies
  • Synthetic biology and pathway engineering
  • Vaccine and antibody development
  • CRISPR/Cas gene editing templates

⭐ Key Benefits

  • Fully customizable gene design
  • Codon optimization for target species
  • Ready-to-use cloned constructs
  • Saves time compared to traditional cloning
About Adaptor

About Adaptor

Named "Adaptor" after the role of the Adaptor in sequencing, we aspire to play a role in bridging and transforming in the fields of biology and information technology. We aim to become the best partner for academia and industry in sample preparation and data analysis, overcoming challenges in this rapidly changing era with the role of an adaptor and creating endless possibilities together.

Development Goals

Adaptor is dedicated to building a "customer-oriented genomic technology integration service platform," providing a series of services from front-end sample preparation and library construction to back-end data processing and analysis. Our aim is to enable the widespread application of new genomic technologies in Taiwan. Currently, through collaboration with Kaohsiung Medical University, we are enhancing the linkage between genomic science and industry applications. This strengthens the integration and completeness of each part of our service platform, allowing us to tailor custom analysis processes to meet both academic and industrial needs. By adopting a "understanding needs, providing solutions" approach, we strive to precisely overcome the challenges in the current bioscience market.
Customized Consultation Services

Customized Consultation Services

We offer genomic technology consultations based on the techniques you wish to apply. This helps you understand the services and analysis results you are about to receive, aligning your project, goals with the services provided by our company, and customizing the genomic services you receive.

Technical Application Consultation

Based on the RAD-seq and RNA genomic technology services provided by our company, we offer pre-experiment consultation and discussion.

Bioinformatics Consultation

Based on the bioinformatics data you obtain, we provide pre-analysis consultation to ensure that the genomic services you use meet your needs.

Customized Data Analysis

We also provide customized analysis for DNA and RNA, which can be tailored according to the references you provide. Analyses include phylogenetic tree construction, genetic structure analysis, and more.

Adaptor Team Introduction

Adaptor Team Introduction

Yi-Fan Chiu

CEO
Director of Bioinformatics

Han-Yun Li

COO
Director of R&D

Yu-Cen Wan

R&D Engineer
Bioinformatics Department

Yi-Yan Li

Data Analyst
Bioinformatics Department
Contact

Contact

Address

80708 Kaohsiung City, Sanmin District, Shiquan Road, No. 100, 12th Floor, Room N1211

Call Us

07-9769339

Email Us

Bioinformatics Department yfchiu@adaptor-genosci.com
Operations Management Department hyli@adaptor-genosci.com

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