Restriction-site Associated DNA Sequencing (RAD-seq) simplifies genomic information using restriction enzymes, increasing the proportion of effective data. It can handle a large number of population samples simultaneously, reducing the research burden. RAD-seq can be applied to evolutionary studies of target species without reference sequences, including population genetic variation, defining genetic differences between individuals, and tracing population phylogenetic and geographic relationships. It is also suitable for establishing Single Nucleotide Polymorphism (SNP) molecular markers, which can be effectively used in agriculture, forestry, fisheries, and other related industries.

• RAD-seq is a simplified genomics technique that combines the advantages of restriction enzyme treatment and high-throughput sequencing to detect and analyze genetic variation between genomes.
• By using restriction enzyme cut sites and fragment lengths as screening criteria, RAD-seq eliminates the need for whole-genome sequencing, reduces sequencing depth requirements, and increases the proportion of effective sequences among similar fragments across samples.
• RAD-seq features the ability to sequence multiple samples simultaneously using DNA barcoding techniques, allowing rapid analysis of genomic variation among individuals/populations and the acquisition of SNP information.
• The technology is widely used in research on genetic diversity in natural populations, species differentiation, mutations, and evolution, as well as in fields such as genome-wide association studies (GWAS) and quantitative trait locus (QTL) mapping.

• **Digestion & Ligation:**
• Use restriction enzymes to cut DNA into smaller fragments. Label each DNA fragment with a specific tag sequence at the cut sites, where each sample has a unique tag sequence to distinguish fragments from different samples.
• **Pooling:**
• Mix DNA fragments from different samples together to form a DNA library.
• **Size Selection:**
• Use size selection to retain fragments of appropriate length for sequencing while removing excessively short fragments.
• **PCR:**
• Amplify DNA fragments using PCR, completing the preparation of the DNA library for NovaSeq high-throughput sequencing.
• **Sequencing:**
• After sequencing, align, assemble, and analyze the sequences to obtain information on genomic variation in the samples and retrieve SNP information.
  
  

You only need to provide DNA samples to receive library construction, sequencing, and bioinformatics assembly services. The assembled data can be directly used for subsequent analyses, eliminating the time and equipment costs associated with pre-experiment setup, data assembly, and formatting issues.

Aida offers derivative analyses after RAD-seq assembly, providing scientific charts that meet specified journal formats. Custom graphing is available according to your needs, highlighting key analytical results, with two rounds of modifications and re-analysis included to ensure the analysis meets your research objectives. We offer services such as Phylogenetic Tree analysis, Population Genetic Structure analysis, and Principal Component Analysis (PCA/DAPC). In addition to the advanced analyses listed above, we also provide customized analysis services based on your reference literature, such as Dispersal Map of Resistance, Fixation Index Analysis, Genome-Wide Association Studies (GWAS), or Quantitative Trait Locus (QTL) Mapping.

Before deciding to use Aida's services, you can contact us for a basic introduction to RAD-seq and a discussion on research and application directions. This will ensure that the services provided by Aida meet your expectations. We also offer sample testing services, where a small quantity of samples is used for enzyme suitability testing to confirm the optimal preparation method for your target samples.